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  • Open Access

    REVIEW

    Advances in PGPR-Mediated Plant-Pathogen Control for Food Security and Ecosystem Stability

    Sajid Ali*, Yong-Sun Moon*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.064284
    (This article belongs to the Special Issue: Multi-Level Mechanisms in Plant-Pathogen Interactions)
    Abstract This review focused on the role of plant growth-promoting rhizobacteria (PGPR) in enhancing plant growth and protecting against pathogens, highlighting their mechanisms of action, ecological benefits, and challenges. PGPR mediate plant growth through several mechanisms, including nutrient acquisition, production of antimicrobial compounds and induction of systemic resistance. These mechanisms are critical in improving crop yields, especially under stressful conditions. This review examines the molecular mechanisms of PGPR-mediated plant pathogen control, cellular mechanisms of PGPR in plant pathogen control, ecological and environmental benefits of PGPR application. Despite their potential, PGPR application is limited by environmental variability,… More >

  • Open Access

    ARTICLE

    Interplay of Temporal Variation in Nectar Parameters and Pollinator-Mediated Adaptations in Epimedium wushanense

    Lanying Chen1,2, Yifu Cai3, Qiumei Quan3,*, Yunxiang Li3
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.064112
    (This article belongs to the Special Issue: Advances in Ornamental Plants: Micropropagation, Plant Biotechnology, Chromosome Doubling, Mutagenesis, Plant Breeding, Environmental Stress Tolerance, and Postharvest Physiology)
    Abstract This study investigates the diurnal patterns of nectar secretion, sugar content, and caloric value in Epimedium wushanense, and their interaction mechanisms with pollinator behavior under varying environmental conditions. Nectar secretion exhibited a diurnal pattern, peaking between 11:00 and 13:00, with progressive increases in both volumes (19.07 ± 1.66 μL/day) and caloric value (6.03 ± 0.55 cal/day) over four consecutive days, culminating in maximal production on Day 4 (p < 0.05). Floral bagging significantly altered nectar traits (Mann-Whitney U test, p < 0.05), with bagged inflorescences demonstrating 61.82% higher nectar volume productivity relative to unbagged controls. Pollinator visitation,… More >

  • Open Access

    A Gel-Free Budget-Friendly Approach to GFP-Tagged Viruses Quantification in Plant Samples

    Rohith Grandhi, Mélodie B. Plourde, Aditi Balasubramani, Hugo Germain*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.063974
    (This article belongs to the Special Issue: Multi-Level Mechanisms in Plant-Pathogen Interactions)
    Abstract Viral diseases are an important threat to crop yield, as they are responsible for losses greater than US$30 billion annually. Thus, understanding the dynamics of virus propagation within plant cells is essential for devising effective control strategies. However, viruses are complex to propagate and quantify. Existing methodologies for viral quantification tend to be expensive and time-consuming. Here, we present a rapid cost-effective approach to quantify viral propagation using an engineered virus expressing a fluorescent reporter. Using a microplate reader, we measured viral protein levels and we validated our findings through comparison by western blot analysis More >

  • Open Access

    ARTICLE

    HPLC-DAD Profiling and Diuretic Effect of Solanum elaeagnifolium (Cav.) Aqueous Extract: A Combined Experimental and Computational Approach

    Bouslamti Mohammed1, Nouioura Ghizlane1, Tbatou Widad1, Mohamed Chebaibi2,3, Abdulsalam Alhalmi4, Najoua Soulo1, Sulaiman Mohammed Alnasser5, Fahad M Alshabrmi6, Amine Elbouzidi7,*, Lyoussi Badiaa1, Benjelloun Ahmed Samir1
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.063896
    (This article belongs to the Special Issue: Innovative Strategies in Medicinal Plant Biotechnology: From Traditional Knowledge to Modern Applications)
    Abstract The Solanum genus is known for its diverse bioactive compounds, yet its diuretic potential remains understudied. This research commenced with an analysis of polyphenol and flavonoid content in Solanum elaeagnifolium leaf extract (SEFE) using colorimetric techniques, followed by HPLC-DAD to delineate its chemical composition. The aqueous extract revealed prominent constituents: naringin (12.38%), quercetin 3-O-B-D-Glucoside (27.25%), and flavone (15.26%). A 15-day study on normal rats investigated the diuretic potential of SEFE at repeated doses. SEFE significantly increased urine volume and urinary sodium/potassium levels without inducing hypokalaemia, contrasting with furosemide, a standard diuretic that induced hypokalaemia. Conversely, furosemide, a… More >

  • Open Access

    ARTICLE

    Ascorbic Acid Alleviates Salt Stress on the Physiology and Growth of Guava Seedlings

    Jackson Silva Nóbrega1,*, Geovani Soares de Lima1, Jean Telvio Andrade Ferreira2, Julio Cesar Agostinho da Silva1, Lauriane Almeida dos Anjos Soares1, Valéria Fernandes de Oliveira Sousa1, Paulo Vinicius de Oliveira Freire1, Reynaldo Teodoro de Fátima1, Flávia de Sousa Almeida1, Hans Raj Gheyi2, Josemir Moura Maia3
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.063633
    (This article belongs to the Special Issue: Abiotic Stress Tolerance in Crop Plants: Physio-biochemical and Molecular Mechanisms)
    Abstract The Northeast region is the main producer of guava in Brazil, generating employment and income. However, water availability means that producer’s resort to using water with high salinity, which harms plant development, especially during the seedling formation phase. The adoption of techniques that mitigate the deleterious effect of salinity is increasingly necessary, such as the use of elicitors such as ascorbic acid. The purpose of this study was to analyze the morphophysiology of guava seedlings under saline and ascorbic acid levels. The study was carried out by applying treatments composed of five saline levels (SL… More >

  • Open Access

    REVIEW

    Zinc Oxide Nanoparticles: Abiotic Stress Tolerance in Fruit Crops Focusing on Sustainable Production

    Meryam Manzoor1, Konstantin Korolev2, Maryam3, Riaz Ahmad4,*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.063930
    (This article belongs to the Special Issue: Advances in Fruit Resistance Research and Applications)
    Abstract The productivity of fruit crops is badly affected by abrupt changes in climatic conditions. It is a matter of concern for fruit tree researchers to feed the huge population within the available resources. The adverse effects of abiotic stresses are increasing due to fluctuations in climate change. Several abiotic stresses (salinity, drought, water logging, minerals deficiency, temperature extremities and heavy metals) are reducing the overall productivity of crops. Therefore, the application of different management approaches, i.e., phytohormones, nanoparticles, organic amendments, microbes and molecular aspects are effective for the mitigation of abiotic stresses in fruit crops.… More >

  • Open Access

    REVIEW

    Systematic Review of Machine Learning Applications in Sustainable Agriculture: Insights on Soil Health and Crop Improvement

    Vicky Anand1, Priyadarshani Rajput1, Tatiana Minkina1, Saglara Mandzhieva1, Santosh Kumar2, Avnish Chauhan3, Vishnu D. Rajput1,*
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.063927
    (This article belongs to the Special Issue: Integrated Nutrient Management in Cereal Crops)
    Abstract The digital revolution in agriculture has introduced data-driven decision-making, where artificial intelligence, especially machine learning (ML), helps analyze large and varied data sources to improve soil quality and crop growth indices. Thus, a thorough evaluation of scientific publications from 2007 to 2024 was conducted via the Scopus and Web of Science databases with the PRISMA guidelines to determine the realistic role of ML in soil health and crop improvement under the SDGs. In addition, the present review focused to identify and analyze the trends, challenges, and opportunities associated with the successful implementation of ML in… More >

  • Open Access

    REVIEW

    Biochar as a Climate-Smart Agricultural Practice: Reducing Greenhouse Gas Emissions and Promoting Sustainable Farming

    Muhammad Nazim1,2,*, Abdul Ghafoor3,*, Abida Hussain4, Mehwish Tabassum5, Aamir Nawaz6, Muhammad Ahmad7, Murad Muhammad1,2, Muqarrab Ali4
    Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.058970
    (This article belongs to the Special Issue: Soil Microbe-Plant Interactions: Unveiling Mechanisms of Biostimulants in Stress Tolerance)
    Abstract In recent years, the world has faced rising global temperatures, accumulative pollution, and energy crises, stimulating scientists worldwide to strive for eco-friendly and cost-effective solutions. Biochar has materialized as a favorable tool for environmental remediation, indicating efficacy as an efficient sorbent substance for both inorganic and organic pollutants in environmental field. These unique properties exclude improved surface functionality, porous morphology, large specific surface area (SSA), cation exchange capacity (CEC), robust adsorption capabilities, environmental stability, and embedded micronutrients. Biochar exhibited potential characteristics for environmental oversight, greenhouse gas (GHG) emission reduction, and soil fertility improvement. This review… More >